ALDEFLUOR™ kit for the identification, evaluation and isolation of stem and progenitor cells expressing high levels of ALDH. ALDEFLUOR&#; Kit from. PRODUCT DESCRIPTION. ALDEFLUOR™ is a reagent kit that is used to identify human cells that express high levels of the enzyme aldehyde dehydrogenase. Here is a quick guide to setting up your FACS analysis with ALDEFLUOR: We’ ve used the Aldefluor kit extensively for both cell lines and for freshly dissociated .
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Lo JF et al. Azzam DJ et al. Frequently Asked Questions The reagents in the kit were frozen when I received it. No antibody staining required. Clin Cancer Res 19 J Neurochem 88 1: Liu L et kih. Pierre-Louis O et al. Yes, but full staining will take at least 3 – 4 hours.
Cancer Res 73 Abstract Despite progress in the development of drugs that efficiently target cancer cells, treatments for metastatic tumours are often ineffective.
Is it acceptable for the staining reaction to exceed 30 minutes? Exp Hematol 35 9: Huang EH et al. In the DEAB-treated control, fluorescence will reflect the size of the intracellular substrate pool. Eur J Cancer 50 1: Aldefpuor rights reserved including graphics and images.
The protocol is scalable and can be used to prepare the large number of cells required for off-the-shelf” therapies and bioengineering applications. Using various strategies to block neutrophil recruitment to the pre-metastatic site, we demonstrate that neutrophils specifically support metastatic initiation.
Yang Y et al. Breast Cancer Cells Alam M et al.
Buijs Klt et al. Brain Tumor Stem Cell Research. Nakamura S et al. Am Heart J 2: Still, despite marked tumour shrinkage, the frequency of relapse indicates that a fraction of tumour cells survives shut down of oncogenic signalling. Flesken-Nikitin A et al.
Cord blood units may be collected into citrate phosphate dextrose anticoagulant. Cancer Res 70 Chen D et al.
Aldeflur is the universal efflux inhibitor. Fluorescence in the test sample will additionally reflect ALDH activity. This transport inhibitor may not prevent efflux from other tissue types or from other species. Mol Cancer Ther 13 4: Lung Cells Moreb JS et al.
It is important to ensure that reagents used for erythrocyte lysis do not contain a fixative. However, the as-yet poorly characterized contribution of host responses during tumour growth and metastatic progression represents a limitation to exploiting this approach.
Clin Cancer Res 18 Pancreatic Cells Rovira M et al. Vazquez-Martin A et al.
ALDEFLUOR™ Kit | STEMCELL Technologies
Safety Data Sheet 5. Hanke M et al.
Liu P et al. Bareiss PM et al.